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Mouse Gnb1 ELISA Kit

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  • Alternative name

    Mouse transducin beta chain 1 ELISA Kit;Mouse AA409223 ELISA Kit;Mouse C77571 ELISA Kit;Mouse Gnb-1 ELISA Kit;Mouse guanine nucleotide binding protein (G protein), beta 1 ELISA Kit;Mouse guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 ELISA Kit;Mouse guanine nucleotide binding protein, beta 1 ELISA Kit;
  • Catalog
    E053575
  • species
    Mouse
  • GeneGnb1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseMouse Gnb1 ELISA Kit allows for the in vitro quantitative determination of Gnb1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse GNb1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse GNb1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse GNb1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse GNb1 can be calculated.
  • Mouse Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Protein information
  • Uniprot ID GBB1_MOUSE
  • Uniprot AC P62874; P04697; P04901;
  • UniGene Mm.2344;
  • GeneID 14688
  • KEGG mmu:14688;
  • Mouse Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Protein SEQUENCE

  • SEQUENCE 340 AA; 37377 MW; 896CBD32D2686598 CRC64;

    MSELDQLRQE AEQLKNQIRD ARKACADATL SQITNNIDPV GRIQMRTRRT

    LRGHLAKIYA MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV

    MTCAYAPSGN YVACGGLDNI CSIYNLKTRE GNVRVSRELA GHTGYLSCCR

    FLDDNQIVTS SGDTTCALWD IETGQQTTTF TGHTGDVMSL SLAPDTRLFV

    SGACDASAKL WDVREGMCRQ TFTGHESDIN AICFFPNGNA FATGSDDATC

    RLFDLRADQE LMTYSHDNII CGITSVSFSK SGRLLLAGYD DFNCNVWDAL

    KADRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN

  • UCSC uc008wdp.2; mouse.;



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