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Mouse G6pc ELISA Kit

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  • Alternative name

    Mouse G-6-Pase ELISA Kit;Mouse G6Pase ELISA Kit;Mouse G6pt ELISA Kit;Mouse AW107337 ELISA Kit;Mouse Glc-6-Pase ELISA Kit;Mouse glucose-6-phosphatase, catalytic ELISA Kit;Mouse glucose-6-phosphatase ELISA Kit;Mouse Glc-6-Pase-alpha ELISA Kit;
  • Catalog
    E053719
  • species
    Mouse
  • GeneG6pc
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse G6PC. No significant cross-reactivity or interference between Mouse G6PC and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse G6PC and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.469ng/mL
  • Intended UseMouse G6pc ELISA Kit allows for the in vitro quantitative determination of G6pc , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of G6PC in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse G6PC. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse G6PC and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse G6PC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse G6PC. You can calculate the concentration of Mouse G6PC in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Glucose-6-phosphatase Protein information
  • Uniprot ID G6PC_MOUSE
  • Uniprot AC P35576; Q91WV3;
  • UniGene Mm.18064;
  • GeneID 14377
  • KEGG mmu:14377;
  • Mouse Glucose-6-phosphatase Protein SEQUENCE

  • SEQUENCE 357 AA; 40473 MW; 292163ACA7A44402 CRC64;

    MEEGMNILHD FGIQSTRYLQ VNYQDSQDWF ILVSVIADLR NAFYVLFPIW

    FHLKETVGIN LLWVAVVGDW FNLVFKWILF GQRPYWWVLD TDYYSNSSVP

    IIKQFPVTCE TGPGSPSGHA MGAAGVYYVM VTSTLAIFRG KKKPTYGFRC

    LNVILWLGFW AVQLNVCLSR IYLAAHFPHQ VVAGVLSGIA VAETFSHIRG

    IYNASLRKYC LITIFLFGFA LGFYLLLKGL GVDLLWTLEK AKRWCERPEW

    VHLDTTPFAS LFKNLGTLLG LGLALNSSMY RKSCKGELSK LLPFRFACIV

    ASLVLLHLFD SLKPPSQVEL IFYILSFCKS ATVPFASVSL IPYCLARILG

    QTHKKSL

  • UCSC uc007lor.1; mouse.;



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