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Mouse Gad2 ELISA Kit

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  • Alternative name

    Mouse 65 kDa glutamic acid decarboxylase ELISA Kit;Mouse GAD-65 ELISA Kit;Mouse glutamate decarboxylase 65 kDa isoform ELISA Kit;Mouse GAD65 ELISA Kit;Mouse 6330404F12Rik ELISA Kit;Mouse GAD(65) ELISA Kit;Mouse Gad-2 ELISA Kit;Mouse glutamic acid decarboxylase 2 ELISA Kit;Mouse glutamate decarboxylase 2 ELISA Kit;
  • Catalog
    E053729
  • species
    Mouse
  • GeneGad2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse GAD2. No significant cross-reactivity or interference between Mouse GAD2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse GAD2 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Gad2 ELISA Kit allows for the in vitro quantitative determination of Gad2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GAD2 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GAD2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse GAD2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GAD2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse GAD2. You can calculate the concentration of Mouse GAD2 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Glutamate decarboxylase 2 Protein information
  • Uniprot ID DCE2_MOUSE
  • Uniprot AC P48320; O35519;
  • UniGene Mm.4784;
  • GeneID 14417
  • KEGG mmu:14417;
  • Mouse Glutamate decarboxylase 2 Protein SEQUENCE

  • SEQUENCE 585 AA; 65224 MW; C2F486E85123B057 CRC64;

    MASPGSGFWS FGSEDGSADP ENPGTARAWC QVAQKFTGGI GNKLCALLYG

    DSGKPAEGGG SVTSRAATGK VACTCDQKPC NCPKGDVNYA FLHATDLLPA

    CDGERPTLAF LQDVMNILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA

    DQPQNLEEIL THCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA

    NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA

    MLIARYKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI

    LIKCDERGKM IPSDLERRIL EVKQKGFVPF LVSATAGTTV YGAFDPLLAV

    ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV

    PLQCSALLVR EEGLMQSCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV

    DVFKLWLMWR AKGTTGFEAH IDKCLELAEY LYTIIKNREG YEMVFDGKPQ

    HTNVCFWFVP PSLRTLEDNE ERMSRLSKVA PVIKARMMEY GTTMVSYQPL

    GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL

  • UCSC uc008inj.1; mouse.;



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