-
Alternative name
Gasdermin-D ELISA KIT; Gasdermin domain-containing protein 1GSDMD ELISA KIT; DFNA5L ELISA KIT; GSDMDC1 ELISA KIT
-
Catalog
E053945
-
species
Mouse
-
GeneGSDMD
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of GSDMD. No significant cross-reactivity or interference between GSDMD and analogues was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity0.063ng/mL.
-
Intended UseMouse GSDMD ELISA Kit allows for the in vitro quantitative determination of GSDMD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalIntended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GSDMD in mouse tissue homogenates and other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to GSDMD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GSDMD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GSDMD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GSDMD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-