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Alternative name
Mouse G71 ELISA Kit;Mouse Gastrin-34 ELISA Kit;Mouse G34 ELISA Kit;Mouse GAS ELISA Kit;Mouse gastrin ELISA Kit;
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Catalog
E053966
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species
Mouse
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GeneGast
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 4.69pg/ml
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Intended UseMouse Gast ELISA Kit allows for the in vitro quantitative determination of Gast , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Gast antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Gast antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Gast amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Gast can be calculated.
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Mouse Gastrin Protein information
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Uniprot ID
GAST_MOUSE
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Uniprot AC
P48757; P70334; Q64295; Q9CPR2;
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UniGene
Mm.4767;
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GeneID
14459
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KEGG
mmu:14459;
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Mouse Gastrin Protein SEQUENCE
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SEQUENCE 101 AA; 11590 MW; 41DE15814DBFB68E CRC64;
MPRLCVYMLV LVLALATFSE ASWKPRSQLQ DASSGPGTNE DLEQRQFNKL
GSASHHRRQL
GPQGPQHFIA DLSKKQRPRM EEEEEAYGWM DFGRRSAEED
Q
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UCSC
uc007lkw.2; mouse.;
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