Mouse Gata3 ELISA Kit

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  • Alternative name

    Mouse GATA-binding factor 3 ELISA Kit;Mouse Gata-3 ELISA Kit;Mouse jal ELISA Kit;Mouse GATA binding protein 3 ELISA Kit;Mouse trans-acting T-cell-specific transcription factor GATA-3 ELISA Kit;

  • Catalog
    E054005
  • species
    Mouse
  • GeneGata3
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 9.4pg/ml
  • Intended UseMouse Gata3 ELISA Kit allows for the in vitro quantitative determination of Gata3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse GATA3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse GATA3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse GATA3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse GATA3 can be calculated.
  • Mouse Trans-acting T-cell-specific transcription factor GATA-3 Protein information
  • Uniprot ID GATA3_MOUSE
  • Uniprot AC P23772;
  • UniGene Mm.313866;
  • GeneID 14462
  • KEGG mmu:14462;
  • Mouse Trans-acting T-cell-specific transcription factor GATA-3 Protein SEQUENCE
  • SEQUENCE 443 AA; 47968 MW; 980E58FB9872E560 CRC64;

    MEVTADQPRW VSHHHPAVLN GQHPDTHHPG LGHSYMEAQY PLTEEVDVLF

    NIDGQGNHVP SYYGNSVRAT VQRYPPTHHG SQVCRPPLLH GSLPWLDGGK

    ALSSHHTASP WNLSPFSKTS IHHGSPGPLS VYPPASSSSL AAGHSSPHLF

    TFPPTPPKDV SPDPSLSTPG SAGSARQDEK ECLKYQVQLP DSMKLETSHS

    RGSMTTLGGA SSSAHHPITT YPPYVPEYSS GLFPPSSLLG GSPTGFGCKS

    RPKARSSTEG RECVNCGATS TPLWRRDGTG HYLCNACGLY HKMNGQNRPL

    IKPKRRLSAA RRAGTSCANC QTTTTTLWRR NANGDPVCNA CGLYYKLHNI

    NRPLTMKKEG IQTRNRKMSS KSKKCKKVHD ALEDFPKSSS FNPAALSRHM

    SSLSHISPFS HSSHMLTTPT PMHPPSGLSF GPHHPSSMVT AMG

  • UCSC uc008ihf.1; mouse.;



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