Mouse Gck ELISA Kit

Alternative name
Mouse hexokinase type IV ELISA Kit;Mouse HK IV ELISA Kit;Mouse hexokinase-4 ELISA Kit;Mouse HK4 ELISA Kit;Mouse hexokinase-D ELISA Kit;Mouse Gk ELISA Kit;Mouse GLK ELISA Kit;Mouse Gls006 ELISA Kit;Mouse HKIV ELISA Kit;Mouse HXKP ELISA Kit;Mouse Hlb62 ELISA Kit;Mouse MODY2 ELISA Kit;Mouse glucokinase ELISA Kit;
Catalog
E054026
species
Mouse
GeneGck
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse GCK. No significant cross-reactivity or interference between Mouse GCK and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse GCK and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.094ng/mL
Intended UseMouse Gck ELISA Kit allows for the in vitro quantitative determination of Gck , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GCK in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GCK. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse GCK and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GCK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse GCK. You can calculate the concentration of Mouse GCK in the samples by comparing the OD of the samples to the standard curve.
Mouse Glucokinase Protein information
Uniprot ID HXK4_MOUSE
Uniprot AC P52792; P52791;
UniGene Mm.220358;
GeneID 103988
KEGG mmu:103988;
Mouse Glucokinase Protein SEQUENCE
SEQUENCE 465 AA; 52089 MW; 8C85EED079A52D61 CRC64;
MLDDRARMEA TKKEKVEQIL AEFQLQEEDL KKVMSRMQKE MDRGLKLETH
QEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEAGQWS
VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF
SFPVRHEDID KGILLNWTKG FKASGAEGNN IVGLLRDAIK RRGDFEMDVV
AMVNDTVATM ISCYYEDRQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR
MCVNTEWGAF GNSGELDEFL LEYDRMVDES SVNPGQQLYE KIIGGKYMGE
LVRLVLLKLV EENLLFHGEA SEQLRTRGAF ETRFVSQVES DSGDRRQILN
ILSTLGLRPS VADCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED
VMRITVGVDG SVYKLHPSFK ERFHASVRRL TPNCEITFIE SEEGSGRGAA
LVSAVACKKA CMLGQ
UCSC uc007hxn.2; mouse. [P52792-1];

Category

Mouse Gck ELISA Kit