Mouse Gtf2h1 ELISA Kit

Alternative name
Mouse basic transcription factor 2 62 kDa subunit ELISA Kit;Mouse BTF2 p62 ELISA Kit;Mouse General transcription factor IIH polypeptide 1 ELISA Kit;Mouse TFIIH basal transcription factor complex p62 subunit ELISA Kit;Mouse 62kDa ELISA Kit;Mouse AW743425 ELISA Kit;Mouse AW822074 ELISA Kit;Mouse C77871 ELISA Kit;Mouse p62 ELISA Kit;Mouse general transcription factor II H, polypeptide 1 ELISA Kit;Mouse general transcription factor IIH subunit 1 ELISA Kit;Mouse BTF2-p62 ELISA Kit;Mouse general transcription factor IIH, polypeptide 1 (62kD subunit) ELISA Kit;
Catalog
E054087
species
Mouse
GeneGtf2h1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseMouse Gtf2h1 ELISA Kit allows for the in vitro quantitative determination of Gtf2h1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse GTF2H1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse GTF2H1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse GTF2H1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse GTF2H1 can be calculated.
Mouse General transcription factor IIH subunit 1 Protein information
Uniprot ID TF2H1_MOUSE
Uniprot AC Q9DBA9; O35637;
UniGene Mm.22700;
GeneID 14884
KEGG mmu:14884;
Mouse General transcription factor IIH subunit 1 Protein SEQUENCE
SEQUENCE 547 AA; 61851 MW; 0167316D11F87969 CRC64;
MATSSEEVLL IVKKVRQKKQ DGALYLMAER IAWAPEGKDR FTISHMYADI
KCQKISPEGK AKIQLQLVLH AGDTTNFHFS NESTAVKERD AVKDLLQQLL
PKFKRKANKE LEEKNRMLQE DPVLFQLYKD LVVSQVISAE EFWANRLNVN
ATDSSTSSHK QDVGISAAFL ADVRPQTDGC NGLRYNLTSD IIESIFRTYP
AVKMKYAETV PHNMTEKEFW TRFFQSHYFH RDRLNTGSKD LFAECAKIDE
KGLKTMVSLG VKNPMLDLTS LEDKPLDEGY SISSVPSTSN SKSIKENSNA
AIIKRFNHHS AMVLAAGLRK QQAQNGQNGE PSSVDGNSGD TDCFQPAVKR
AKLQESIEYE DLGNNNSVKT IALNLKKSDR YYHGPTPIQS LQYATSQDII
NSFQSIRQEM EAYTPKLTQV LSSSAASSTI TALSPGGALM QGGTQQAVNQ
MVPNDIQSEL KHLYVAVGEL LRHFWSCFPV NTPFLEEKVV KMKSNLERFQ
VTKLCPFQEK IRRQYLSTNL VSHIEEMLQT AYNKLHTWQS RRLMKKT

Category

Mouse Gtf2h1 ELISA Kit