Mouse Gstt2 ELISA Kit

Alternative name
Mouse GST class-theta-2 ELISA Kit;Mouse AI266894 ELISA Kit;Mouse Yrs ELISA Kit;Mouse mGSTT2 ELISA Kit;Mouse glutathione S-transferase, theta 2 ELISA Kit;Mouse glutathione S-transferase theta-2 ELISA Kit;
Catalog
E054511
species
Mouse
GeneGstt2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Gstt2 ELISA Kit allows for the in vitro quantitative determination of Gstt2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: GSTT2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GSTT2 antibody and an GSTT2-HRP conjugate. The assay sample and buffer are incubated together with GSTT2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSTT2 concentration since GSTT2 from samples and GSTT2-HRP conjugate compete for the anti-GSTT2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GSTT2 from the sample, fewer sites are left to bind GSTT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSTT2 concentration in each sample is interpolated from this standard curve.
Mouse Glutathione S-transferase theta-2 Protein information
Uniprot ID GSTT2_MOUSE
Uniprot AC Q61133; Q61134; Q64472; Q91VB0;
UniGene Mm.24118;
GeneID 14872
KEGG mmu:14872;
Mouse Glutathione S-transferase theta-2 Protein SEQUENCE
SEQUENCE 244 AA; 27634 MW; 3F45D6E12DA3DF29 CRC64;
MGLELYLDLL SQPSRAVYIF AKKNGIPFQT RTVDILKGQH MSEQFSQVNC
LNKVPVLKDG SFVLTESTAI LIYLSSKYQV ADHWYPADLQ ARAQVHEYLG
WHADNIRGTF GVLLWTKVLG PLIGVQVPQE KVERNRDRMV LVLQQLEDKF
LRDRAFLVGQ QVTLADLMSL EELMQPVALG YNLFEGRPQL TAWRERVEAF
LGAELCQEAH STILSILGQA AKKMLPVPPP EVHASMQLRI ARIP
UCSC uc011xgs.1; mouse.;

Category

Mouse Gstt2 ELISA Kit

Mouse GST class-theta-2 ELISA Kit;Mouse AI266894 ELISA Kit;Mouse Yrs ELISA Kit;Mouse mGSTT2 ELISA Kit;Mouse glutathione S-transferase, theta 2 ELISA Kit;Mouse glutathione S-transferase theta-2 ELISA Kit;