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Alternative name
Glycoprotein endo-alpha-1,2-mannosidase ELISA KIT; Manea ELISA KIT; Endo-alpha mannosidase ELISA KIT; Endomannosidase ELISA KIT; mEndo ELISA KIT
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Catalog
E054644
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species
Mouse
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GeneManea
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SpecificityThis assay recognizes recombinant and natural mouse Endo-alpha mannosidase. No significant cross-reactivity or interference was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseMouse Manea ELISA Kit allows for the in vitro quantitative determination of Manea , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endo-alpha mannosidase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Endo-alpha mannosidase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Endo-alpha mannosidase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Endo-alpha mannosidase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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