Mouse Gpc4 ELISA Kit

Alternative name
Mouse K-glypican ELISA Kit;Mouse 9530073D23Rik ELISA Kit;Mouse AF311610 ELISA Kit;Mouse AI385680 ELISA Kit;Mouse AI661372 ELISA Kit;Mouse glypican 4 ELISA Kit;Mouse glypican-4 ELISA Kit;
Catalog
E054698
species
Mouse
GeneGpc4
SpecificityThis assay has high sensitivity and excellent specificity for detection of GPC4. No significant cross-reactivity or interference between GPC4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GPC4 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseMouse Gpc4 ELISA Kit allows for the in vitro quantitative determination of Gpc4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the assay: GPC4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GPC4 antibody and an GPC4-HRP conjugate. The assay sample and buffer are incubated together with GPC4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPC4 concentration since GPC4 from samples and GPC4-HRP conjugate compete for the anti-GPC4 antibody binding site. Since the number of sites is limited, as more sites are occupied by GPC4 from the sample, fewer sites are left to bind GPC4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPC4 concentration in each sample is interpolated from this standard curve.
Mouse Secreted glypican-4 Protein information
Uniprot ID GPC4_MOUSE
Uniprot AC P51655; Q923C8;
UniGene Mm.1528; Mm.478164; Mm.491492;
GeneID 14735
KEGG mmu:14735;
Mouse Secreted glypican-4 Protein SEQUENCE
SEQUENCE 557 AA; 62586 MW; F216AA0FEF5ED840 CRC64;
MARLGLLALL CTLAALSASL LAAELKSKSC SEVRRLYVSK GFNKNDAPLY
EINGDHLKIC PQDYTCCSQE MEEKYSLQSK DDFKTVVSEQ CNHLQAIFAS
RYKKFDEFFK ELLENAEKSL NDMFVKTYGH LYMQNSELFK DLFVELKRYY
VAGNVNLEEM LNDFWARLLE RMFRLVNSQY HFTDEYLECV SKYTEQLKPF
GDVPRKLKLQ VTRAFVAART FAQGLAVARD VVSKVSVVNP TAQCTHALLK
MIYCSHCRGL VTVKPCYNYC SNIMRGCLAN QGDLDFEWNN FIDAMLMVAE
RLEGPFNIES VMDPIDVKIS DAIMNMQDNS VQVSQKVFQG CGPPKPLPAG
RISRSISESA FSARFRPYHP EQRPTTAAGT SLDRLVTDVK EKLKQAKKFW
SSLPSTVCND ERMAAGNENE DDCWNGKGKS RYLFAVTGNG LANQGNNPEV
QVDTSKPDIL ILRQIMALRV MTSKMKNAYN GNDVDFFDIS DESSGEGSGS
GCEYQQCPSE FEYNATDHSG KSANEKADSA GGAHAEAKPY LLAALCILFL
AVQGEWR
UCSC uc012hhb.1; mouse.;

Category

Mouse Gpc4 ELISA Kit

Mouse K-glypican ELISA Kit;Mouse 9530073D23Rik ELISA Kit;Mouse AF311610 ELISA Kit;Mouse AI385680 ELISA Kit;Mouse AI661372 ELISA Kit;Mouse glypican 4 ELISA Kit;Mouse glypican-4 ELISA Kit;