Mouse GnRH ELISA Kit

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  • Catalog
    E054735
  • species
    Mouse
  • GeneGnRH
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Gonadotropin Releasing Hormone (GnRH) No significant cross-reactivity or interference between Gonadotropin Releasing Hormone (GnRH) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 4.62pg/mL.
  • Intended UseMouse GnRH ELISA Kit allows for the in vitro quantitative determination of GnRH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific for Gonadotropin Releasing Hormone (GnRH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Gonadotropin Releasing Hormone (GnRH) and unlabeled Gonadotropin Releasing Hormone (GnRH) (Standards or samples) with the pre-coated antibody specific for Gonadotropin Releasing Hormone (GnRH). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Gonadotropin Releasing Hormone (GnRH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gonadotropin Releasing Hormone (GnRH) in the sample.



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