Mouse Grb7 ELISA Kit

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  • Alternative name

    Mouse epidermal growth factor receptor GRB-7 ELISA Kit;Mouse GRB7 adapter protein ELISA Kit;Mouse mKIAA4028 ELISA Kit;Mouse growth factor receptor bound protein 7 ELISA Kit;Mouse growth factor receptor-bound protein 7 ELISA Kit;Mouse Growth factor receptor-bound protein 7 (GRB7 adapter protein) (Epidermal growth factor receptor GRB-7) ELISA Kit;

  • Catalog
    E054835
  • species
    Mouse
  • GeneGrb7
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse Grb7. No significant cross-reactivity or interference between Mouse Grb7 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse Grb7 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Grb7 ELISA Kit allows for the in vitro quantitative determination of Grb7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Grb7 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Grb7. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse Grb7 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Grb7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse Grb7. You can calculate the concentration of Mouse Grb7 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Growth factor receptor-bound protein 7 Protein information
  • Uniprot ID GRB7_MOUSE
  • Uniprot AC Q03160; Q3TH55;
  • UniGene Mm.276702;
  • GeneID 14786
  • KEGG mmu:14786;
  • Mouse Growth factor receptor-bound protein 7 Protein SEQUENCE
  • SEQUENCE 535 AA; 59959 MW; CD8C307864703645 CRC64;

    MELDLSPTHL SSSPEDVCPT PATPPETPPP PDNPPPGDVK RSQPLPIPSS

    RKLREEEFQA TSLPSIPNPF PELCSPPSQK PILGGSSGAR GLLPRDSSRL

    CVVKVYSEDG ACRSVEVAAG ATARHVCEML VQRAHALSDE SWGLVESHPY

    LALERGLEDH EFVVEVQEAW PVGGDSRFIF RKNFAKYELF KSPPHTLFPE

    KMVSSCLDAQ TGISHEDLIQ NFLNAGSFPE IQGFLQLRGS GRGSGRKLWK

    RFFCFLRRSG LYYSTKGTSK DPRHLQYVAD VNESNVYVVT QGRKLYGMPT

    DFGFCVKPNK LRNGHKGLHI FCSEDEQSRT CWLAAFRLFK YGVQLYKNYQ

    QAQSRHLRLS YLGSPPLRSV SDNTLVAMDF SGHAGRVIDN PREALSAAME

    EAQAWRKKTN HRLSLPTTCS GSSLSAAIHR TQPWFHGRIS REESQRLIGQ

    QGLVDGVFLV RESQRNPQGF VLSLCHLQKV KHYLILPSED EGCLYFSMDE

    GQTRFTDLLQ LVEFHQLNRG ILPCLLRHCC ARVAL

  • UCSC uc007lgk.1; mouse.;



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