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Alternative name
Heat shock protein 60, mitochondrial ELISA KIT; Antigen HIS-62HSP60 ELISA KIT;
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Catalog
E055297
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species
Mouse
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GeneHSP60 Ab
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SpecificityThis assay has high sensitivity and excellent specificity for detection of HSP-Ab. No significant cross-reactivity or interference between HSP-Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSP-Ab and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 ng/mL
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Intended UseMouse HSP60 Ab ELISA Kit allows for the in vitro quantitative determination of HSP60 Ab , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilySignal Transduction
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Product Description
specificalPrinciple of the assay: HSP-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing HSP antigen and an HSP-Ab-HRP conjugate. The assay sample and buffer are incubated together with HSP-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP-Ab concentration since HSP-Ab from samples and HSP-Ab-HRP conjugate compete for the HSP antigen binding site. Since the number of sites is limited, as more sites are occupied by HSP-Ab from the sample, fewer sites are left to bind HSP-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP-Ab concentration in each sample is interpolated from this standard curve.
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