Mouse Ihh ELISA Kit

Alternative name
Mouse IHH ELISA Kit;Mouse HHG-2 ELISA Kit;Mouse Indian hedgehog ELISA Kit;Mouse indian hedgehog protein ELISA Kit;
Catalog
E055361
species
Mouse
GeneIhh
SpecificityThis assay has high sensitivity and excellent specificity for detection of IHH. No significant cross-reactivity or interference between IHH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IHH and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Ihh ELISA Kit allows for the in vitro quantitative determination of Ihh , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: IHH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IHH antibody and an IHH-HRP conjugate. The assay sample and buffer are incubated together with IHH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IHH concentration since IHH from samples and IHH-HRP conjugate compete for the anti-IHH antibody binding site. Since the number of sites is limited, as more sites are occupied by IHH from the sample, fewer sites are left to bind IHH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IHH concentration in each sample is interpolated from this standard curve.
Mouse Indian hedgehog protein C-product Protein information
Uniprot ID IHH_MOUSE
Uniprot AC P97812; Q61724;
UniGene Mm.439736;
GeneID 16147
KEGG mmu:16147;
Mouse Indian hedgehog protein C-product Protein SEQUENCE
SEQUENCE 411 AA; 45485 MW; 08BE7AD8507C0D9B CRC64;
MSPAWLRPRL RFCLFLLLLL LVPAARGCGP GRVVGSRRRP PRKLVPLAYK
QFSPNVPEKT LGASGRYEGK IARSSERFKE LTPNYNPDII FKDEENTGAD
RLMTQRCKDR LNSLAISVMN QWPGVKLRVT EGWDEDGHHS EESLHYEGRA
VDITTSDRDR NKYGLLARLA VEAGFDWVYY ESKAHVHCSV KSEHSAAAKT
GGCFPAGAQV RLENGERVAL SAVKPGDRVL AMGEDGTPTF SDVLIFLDRE
PNRLRAFQVI ETQDPPRRLA LTPAHLLFIA DNHTEPAAHF RATFASHVQP
GQYVLVSGVP GLQPARVAAV STHVALGSYA PLTRHGTLVV EDVVASCFAA
VADHHLAQLA FWPLRLFPSL AWGSWTPSEG VHWYPQMLYR LGRLLLEEST
FHPLGMSGAG S

Category

Mouse Ihh ELISA Kit

Mouse IHH ELISA Kit;Mouse HHG-2 ELISA Kit;Mouse Indian hedgehog ELISA Kit;Mouse indian hedgehog protein ELISA Kit;