Mouse Shh ELISA Kit

Alternative name
Mouse SHH ELISA Kit;Mouse HHG-1 ELISA Kit;Mouse Sonic hedgehog protein 19 kDa product ELISA Kit;Mouse Sonic hedgehog protein 27 kDa product ELISA Kit;Mouse Hhg1 ELISA Kit;Mouse 9530036O11Rik ELISA Kit;Mouse Dsh ELISA Kit;Mouse Hx ELISA Kit;Mouse Hxl3 ELISA Kit;Mouse M100081 ELISA Kit;Mouse sonic hedgehog ELISA Kit;Mouse sonic hedgehog protein ELISA Kit;Mouse hemimelic extra toes ELISA Kit;Mouse short digits ELISA Kit;
Catalog
E055364
species
Mouse
GeneShh
SpecificityThis assay has high sensitivity and excellent specificity for detection of SHH. No significant cross-reactivity or interference between SHH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SHH and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseMouse Shh ELISA Kit allows for the in vitro quantitative determination of Shh , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: SHH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SHH. Standards or samples are then added to the microtiter plate wells and SHH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SHH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SHH are added to each well to "sandwich" the SHH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SHH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SHH concentration in each sample is interpolated from this standard curve.
Mouse Sonic hedgehog protein C-product Protein information
Uniprot ID SHH_MOUSE
Uniprot AC Q62226;
UniGene Mm.57202;
GeneID 20423
KEGG mmu:20423;
Mouse Sonic hedgehog protein C-product Protein SEQUENCE
SEQUENCE 437 AA; 47773 MW; D0EB72F08E7860EF CRC64;
MLLLLARCFL VILASSLLVC PGLACGPGRG FGKRRHPKKL TPLAYKQFIP
NVAEKTLGAS GRYEGKITRN SERFKELTPN YNPDIIFKDE ENTGADRLMT
QRCKDKLNAL AISVMNQWPG VKLRVTEGWD EDGHHSEESL HYEGRAVDIT
TSDRDRSKYG MLARLAVEAG FDWVYYESKA HIHCSVKAEN SVAAKSGGCF
PGSATVHLEQ GGTKLVKDLR PGDRVLAADD QGRLLYSDFL TFLDRDEGAK
KVFYVIETLE PRERLLLTAA HLLFVAPHND SGPTPGPSAL FASRVRPGQR
VYVVAERGGD RRLLPAAVHS VTLREEEAGA YAPLTAHGTI LINRVLASCY
AVIEEHSWAH RAFAPFRLAH ALLAALAPAR TDGGGGGSIP AAQSATEARG
AEPTAGIHWY SQLLYHIGTW LLDSETMHPL GMAVKSS
UCSC uc008wua.2; mouse.;

Category

Mouse Shh ELISA Kit