Mouse Hgf ELISA Kit

Alternative name
Mouse hepatopoietin-A ELISA Kit;Mouse scatter factor ELISA Kit;Mouse SF ELISA Kit;Mouse C230052L06Rik ELISA Kit;Mouse HGF/SF ELISA Kit;Mouse NK1 ELISA Kit;Mouse NK2 ELISA Kit;Mouse SF/HGF ELISA Kit;Mouse hepatocyte growth factor ELISA Kit;
Catalog
E055571
species
Mouse
GeneHgf
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Hgf ELISA Kit allows for the in vitro quantitative determination of Hgf , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the Assay: HGF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HGF. Standards or samples are then added to the microtiter plate wells and HGF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HGF present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for HGF are added to each well to "sandwich" the HGF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HGF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HGF concentration in each sample is interpolated from this standard curve.
Mouse Hepatocyte growth factor beta chain Protein information
Uniprot ID HGF_MOUSE
Uniprot AC Q08048; O55027; Q53WS5; Q61662; Q64007; Q6LBE6;
UniGene Mm.267078;
GeneID 15234
KEGG mmu:15234;
Mouse Hepatocyte growth factor beta chain Protein SEQUENCE
SEQUENCE 728 AA; 82945 MW; A0381FC497534328 CRC64;
MMWGTKLLPV LLLQHVLLHL LLLHVAIPYA EGQKKRRNTL HEFKKSAKTT
LTKEDPLLKI KTKKVNSADE CANRCIRNRG FTFTCKAFVF DKSRKRCYWY
PFNSMSSGVK KGFGHEFDLY ENKDYIRNCI IGKGGSYKGT VSITKSGIKC
QPWNSMIPHE HSFLPSSYRG KDLQENYCRN PRGEEGGPWC FTSNPEVRYE
VCDIPQCSEV ECMTCNGESY RGPMDHTESG KTCQRWDQQT PHRHKFLPER
YPDKGFDDNY CRNPDGKPRP WCYTLDPDTP WEYCAIKTCA HSAVNETDVP
METTECIQGQ GEGYRGTSNT IWNGIPCQRW DSQYPHKHDI TPENFKCKDL
RENYCRNPDG AESPWCFTTD PNIRVGYCSQ IPKCDVSSGQ DCYRGNGKNY
MGNLSKTRSG LTCSMWDKNM EDLHRHIFWE PDASKLNKNY CRNPDDDAHG
PWCYTGNPLI PWDYCPISRC EGDTTPTIVN LDHPVISCAK TKQLRVVNGI
PTQTTVGWMV SLKYRNKHIC GGSLIKESWV LTARQCFPAR NKDLKDYEAW
LGIHDVHERG EEKRKQILNI SQLVYGPEGS DLVLLKLARP AILDNFVSTI
DLPSYGCTIP EKTTCSIYGW GYTGLINADG LLRVAHLYIM GNEKCSQHHQ
GKVTLNESEL CAGAEKIGSG PCEGDYGGPL ICEQHKMRMV LGVIVPGRGC
AIPNRPGIFV RVAYYAKWIH KVILTYKL
UCSC uc008wnj.1; mouse. [Q08048-2];

Category

Mouse Hgf ELISA Kit

Mouse hepatopoietin-A ELISA Kit;Mouse scatter factor ELISA Kit;Mouse SF ELISA Kit;Mouse C230052L06Rik ELISA Kit;Mouse HGF/SF ELISA Kit;Mouse NK1 ELISA Kit;Mouse NK2 ELISA Kit;Mouse SF/HGF ELISA Kit;Mouse hepatocyte growth factor ELISA Kit;