Mouse Hnf4a ELISA Kit

Alternative name
Mouse HNF-4-alpha ELISA Kit;Mouse nuclear receptor subfamily 2 group A member 1 ELISA Kit;Mouse transcription factor 14 ELISA Kit;Mouse TCF-14 ELISA Kit;Mouse transcription factor HNF-4 ELISA Kit;Mouse HNF-4 ELISA Kit;Mouse Hnf4 ELISA Kit;Mouse Nr2a1 ELISA Kit;Mouse Tcf14 ELISA Kit;Mouse Hnf4alpha ELISA Kit;Mouse MODY1 ELISA Kit;Mouse hepatic nuclear factor 4, alpha ELISA Kit;Mouse hepatocyte nuclear factor 4-alpha ELISA Kit;Mouse HNF4 alpha ELISA Kit;Mouse Nuclear receptor 2A1 ELISA Kit;
Catalog
E055596
species
Mouse
GeneHnf4a
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<23.4 pg/mL
Intended UseMouse Hnf4a ELISA Kit allows for the in vitro quantitative determination of Hnf4a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse HNF4a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse HNF4a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse HNF4a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse HNF4a can be calculated.
Mouse Hepatocyte nuclear factor 4-alpha Protein information
Uniprot ID HNF4A_MOUSE
Uniprot AC P49698; A2A5I5; A2ICH0; Q3UNX3; Q8CFY1;
UniGene Mm.202383;
GeneID 15378
KEGG mmu:15378;
Mouse Hepatocyte nuclear factor 4-alpha Protein SEQUENCE
SEQUENCE 474 AA; 52684 MW; 10843D528EAE87EE CRC64;
MRLSKTLAGM DMADYSAALD PAYTTLEFEN VQVLTMGNDT SPSEGANLNS
SNSLGVSALC AICGDRATGK HYGASSCDGC KGFFRRSVRK NHMYSCRFSR
QCVVDKDKRN QCRYCRLKKC FRAGMKKEAV QNERDRISTR RSSYEDSSLP
SINALLQAEV LSQQITSPIS GINGDIRAKK IANITDVCES MKEQLLVLVE
WAKYIPAFCE LLLDDQVALL RAHAGEHLLL GATKRSMVFK DVLLLGNDYI
VPRHCPELAE MSRVSIRILD ELVLPFQELQ IDDNEYACLK AIIFFDPDAK
GLSDPGKIKR LRSQVQVSLE DYINDRQYDS RGRFGELLLL LPTLQSITWQ
MIEQIQFIKL FGMAKIDNLL QEMLLGGSAS DAPHTHHPLH PHLMQEHMGT
NVIVANTMPS HLSNGQMCEW PRPRGQAATP ETPQPSPPSG SGSESYKLLP
GAITTIVKPP SAIPQPTITK QEAI
UCSC uc012cit.1; mouse. [P49698-2];

Category

Mouse Hnf4a ELISA Kit