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Alternative name
Hepcidin ELISA KIT; HAMP ELISA KIT; HEPC ELISA KIT
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Catalog
E055614
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species
Mouse
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GeneHepc
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SpecificityThis assay has high sensitivity and excellent specificity for detection of hepcidin. No significant cross-reactivity or interference between hepcidin and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.55ng/mL.
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Intended UseMouse Hepc ELISA Kit allows for the in vitro quantitative determination of Hepc , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of hepcidin in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay||This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to hepcidin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled hepcidin and unlabeled hepcidin (Standards or samples) with the pre-coated antibody specific to hepcidin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of hepcidin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of hepcidin in the sample.
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