Mouse Hif1a ELISA Kit

Alternative name
Mouse HIF-1-alpha ELISA Kit;Mouse HIF1-alpha ELISA Kit;Mouse ARNT-interacting protein ELISA Kit;Mouse AA959795 ELISA Kit;Mouse HIF1alpha ELISA Kit;Mouse MOP1 ELISA Kit;Mouse bHLHe78 ELISA Kit;Mouse hypoxia inducible factor 1, alpha subunit ELISA Kit;Mouse hypoxia-inducible factor 1-alpha ELISA Kit;
Catalog
E056172
species
Mouse
GeneHif1a
SpecificityThis assay has high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 1 Alpha (HIF1a). No significant cross-reactivity or interference between Hypoxia Inducible Factor 1 Alpha (HIF1a) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.061ng/mL.
Intended UseMouse Hif1a ELISA Kit allows for the in vitro quantitative determination of Hif1a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 1 Alpha (HIF1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxia Inducible Factor 1 Alpha (HIF1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxia Inducible Factor 1 Alpha (HIF1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hypoxia Inducible Factor 1 Alpha (HIF1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Hypoxia-inducible factor 1-alpha Protein information
Uniprot ID HIF1A_MOUSE
Uniprot AC Q8R385; Q9CYA8;
UniGene Mm.3879; Mm.446610;
GeneID 15251
KEGG mmu:15251;
Mouse Hypoxia-inducible factor 1-alpha Protein SEQUENCE
SEQUENCE 836 AA; 93516 MW; 84D64ECAC2CC753B CRC64;
MEGAGGENEK KKMSSERRKE KSRDAARSRR SKESEVFYEL AHQLPLPHNV
SSHLDKASVM RLTISYLRVR KLLDAGGLDS EDEMKAQMDC FYLKALDGFV
MVLTDDGDMV YISDNVNKYM GLTQFELTGH SVFDFTHPCD HEEMREMLTH
RNGPVRKGKE LNTQRSFFLR MKCTLTSRGR TMNIKSATWK VLHCTGHIHV
YDTNSNQPQC GYKKPPMTCL VLICEPIPHP SNIEIPLDSK TFLSRHSLDM
KFSYCDERIT ELMGYEPEEL LGRSIYEYYH ALDSDHLTKT HHDMFTKGQV
TTGQYRMLAK RGGYVWVETQ ATVIYNTKNS QPQCIVCVNY VVSGIIQHDL
IFSLQQTESV LKPVESSDMK MTQLFTKVES EDTSCLFDKL KKEPDALTLL
APAAGDTIIS LDFGSDDTET EDQQLEDVPL YNDVMFPSSN EKLNINLAMS
PLPSSETPKP LRSSADPALN QEVALKLESS PESLGLSFTM PQIQDQPASP
SDGSTRQSSP ERLLQENVNT PNFSQPNSPS EYCFDVDSDM VNVFKLELVE
KLFAEDTEAK NPFSTQDTDL DLEMLAPYIP MDDDFQLRSF DQLSPLESNS
PSPPSMSTVT GFQQTQLQKP TITATATTTA TTDESKTETK DNKEDIKILI
ASPSSTQVPQ ETTTAKASAY SGTHSRTASP DRAGKRVIEQ TDKAHPRSLN
LSATLNQRNT VPEEELNPKT IASQNAQRKR KMEHDGSLFQ AAGIGTLLQQ
PGDCAPTMSL SWKRVKGFIS SEQNGTEQKT IILIPSDLAC RLLGQSMDES
GLPQLTSYDC EVNAPIQGSR NLLQGEELLR ALDQVN
UCSC uc007nwq.2; mouse. [Q61221-1];

Category

Mouse Hif1a ELISA Kit

Mouse HIF-1-alpha ELISA Kit;Mouse HIF1-alpha ELISA Kit;Mouse ARNT-interacting protein ELISA Kit;Mouse AA959795 ELISA Kit;Mouse HIF1alpha ELISA Kit;Mouse MOP1 ELISA Kit;Mouse bHLHe78 ELISA Kit;Mouse hypoxia inducible factor 1, alpha subunit ELISA Kit;Mouse hypoxia-inducible factor 1-alpha ELISA Kit;