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Alternative name
Hypoxia-inducible factor 1-alpha ELISA KIT; ARNT-interacting protein ELISA KIT; Basic-helix-loop-helix-PAS protein MOP1 ELISA KIT; Class E basic helix-loop-helix protein 78 ELISA KIT; bHLHe78 ELISA KIT; Member of PAS protein 1 ELISA KIT; PAS domain-containing protein 8HIF1A ELISA KIT; BHLHE78 ELISA KIT; MOP1 ELISA KIT; PASD8 ELISA KIT; HIF-1-alpha ELISA KIT; HIF1-alpha ELISA KIT; bHLHe78 ELISA KIT
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Catalog
E056179
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species
Mouse
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GeneHif1alpha
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 75pg/ml
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Intended UseMouse Hif1alpha ELISA Kit allows for the in vitro quantitative determination of Hif1alpha , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Hif1alphaantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Hif1alpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Hif1alpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofMouse Hif1alphacan be calculated.
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