Mouse Ids ELISA Kit

Alternative name
Mouse alpha-L-iduronate sulfate sulfatase ELISA Kit;Mouse AW214631 ELISA Kit;Mouse iduronate 2-sulfatase ELISA Kit;Mouse iduronate sulfatase ELISA Kit;
Catalog
E056197
species
Mouse
GeneIds
SpecificityNatural and recombinant mouse IDS
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Sample For quantitative detection of mouse IDS in cell culture supernates, serum and plasma
Intended UseMouse Ids ELISA Kit allows for the in vitro quantitative determination of Ids , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: Mouse IDS ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IDS has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: T36-P552) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IDS is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IDS amount of sample captured in plate. Background: Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Mouse Iduronate 2-sulfatase Protein information
Uniprot ID IDS_MOUSE
Uniprot AC Q08890; Q32KI7; Q3TM30;
UniGene Mm.233083;
GeneID 15931
KEGG mmu:15931;
Mouse Iduronate 2-sulfatase Protein SEQUENCE
SEQUENCE 552 AA; 62186 MW; 3E3D819A823AFDE5 CRC64;
MSPPPPPPIW RQLSFSLLLG SFCIALESAA QGNSATDALN ILLIIVDDLR
PSLGCYGDKL VRSPNIDQLA SHSVLFQNAF AQQAVCAPSR VSFLTGRRPD
TTRLYDFNSY WRVHSGNFST IPQYFKENGY VTMSVGKVFH PGISSNHSDD
YPYSWSFPPY HPSSEKYENT KTCKGQDGKL HANLLCPVDV ADVPEGTLPD
KQSTEEAIRL LEKMKTSASP FFLAVGYHKP HIPFRYPKEF QKLYPLENIT
LAPDPHVPDS LPPVAYNPWM DIREREDVQA LNISVPYGPI PEDFQRKIRQ
SYFASVSYLD TQVGHVLSAL DDLRLAHNTI IAFTSDHGWA LGEHGEWAKY
SNFDVATRVP LMLYVPGRTA PLPAAGQKLF PYRDPFDPAS DWMDAGRHTE
DLVELVSLFP TLAGLAGLPV PPRCPIPSFH VELCREGQNL QKHLQLHDLE
EEPDLFGNPR ELIAYSQYPR PADFPQWNSD KPSLNDIKVM GYSIRTVDYR
YTVWVGFDPS EFLANFSDIH AGELYFVDSD PLQDHNVYND SQHGGLLHSL
RP
UCSC uc012hjl.1; mouse.;

Category

Mouse Ids ELISA Kit

Mouse alpha-L-iduronate sulfate sulfatase ELISA Kit;Mouse AW214631 ELISA Kit;Mouse iduronate 2-sulfatase ELISA Kit;Mouse iduronate sulfatase ELISA Kit;