Mouse Igfbp2 ELISA Kit

Alternative name
Mouse IBP-2 ELISA Kit;Mouse IGF-binding protein 2 ELISA Kit;Mouse Igfbp-2 ELISA Kit;Mouse mIGFBP-2 ELISA Kit;Mouse AI255832 ELISA Kit;Mouse insulin-like growth factor binding protein 2 ELISA Kit;Mouse insulin-like growth factor-binding protein 2 ELISA Kit;
Catalog
E056236
species
Mouse
GeneIgfbp2
SpecificityNatural and recombinant mouse IGFBP2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseMouse Igfbp2 ELISA Kit allows for the in vitro quantitative determination of Igfbp2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse IGFBP2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP2 has been precoated onto 96-well plates. Standards(NSO, E35-Q305) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGFBP2 amount of sample captured in plate. Background: Insulin-like growth factor-binding protein 2(IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.
Mouse Insulin-like growth factor-binding protein 2 Protein information
Uniprot ID IBP2_MOUSE
Uniprot AC P47877; Q61722; Q91VK7;
UniGene Mm.141936;
GeneID 16008
KEGG mmu:16008;
Mouse Insulin-like growth factor-binding protein 2 Protein SEQUENCE
SEQUENCE 305 AA; 32847 MW; 179FE6A304E93A16 CRC64;
MLPRLGGPAL PLLLPSLLLL LLLGAGGCGP GVRAEVLFRC PPCTPERLAA
CGPPPDAPCA ELVREPGCGC CSVCARQEGE ACGVYIPRCA QTLRCYPNPG
SELPLKALVT GAGTCEKRRV GTTPQQVADS DDDHSEGGLV ENHVDGTMNM
LGGGSSAGRK PLKSGMKELA VFREKVNEQH RQMGKGAKHL SLEEPKKLRP
PPARTPCQQE LDQVLERIST MRLPDDRGPL EHLYSLHIPN CDKHGRYNLK
QCKMSLNGQR GECWCVNPNT GKPIQGAPTI RGDPECHLFY NEQQETGGAH
AQSVQ
UCSC uc007bkw.2; mouse.;

Category

Mouse Igfbp2 ELISA Kit

Mouse IBP-2 ELISA Kit;Mouse IGF-binding protein 2 ELISA Kit;Mouse Igfbp-2 ELISA Kit;Mouse mIGFBP-2 ELISA Kit;Mouse AI255832 ELISA Kit;Mouse insulin-like growth factor binding protein 2 ELISA Kit;Mouse insulin-like growth factor-binding protein 2 ELISA Kit;