SpecificityThe Mouse IL-20 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse IL-20 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
Intended UseMouse Il20 ELISA Kit allows for the in vitro quantitative determination of Il20 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay||The Mouse IL-20 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-20 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse IL-20 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-20 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-20 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-20 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: IL-20, also known as Interleukin 20 or Zcyto10, is a protein that in humans is encoded by the IL20 gene. This gene is mapped to 1q32.1. IL-20 is a protein belonging to the IL-10 family of cytokines. This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.