Mouse Il6 ELISA Kit

Alternative name
Mouse Il-6 ELISA Kit;Mouse B-cell hybridoma growth factor ELISA Kit;Mouse interleukin HP-1 ELISA Kit;Mouse interleukin 6 ELISA Kit;Mouse interleukin-6 ELISA Kit;
Catalog
E056385
species
Mouse
GeneIl6
SpecificityNatural and recombinant mouse IL-6
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 1pg/ml
Intended UseMouse Il6 ELISA Kit allows for the in vitro quantitative determination of Il6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse IL-6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-6 has been precoated onto 96-well plates. Standards(E.coli, F25-T211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-6 amount of sample captured in plate. Background: The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells.1 Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factorbecause it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency.2 IL-6 has many roles essential to the regulation of the immuneresponse, hematopoiesis, and bone resorption.3 It is involved not only in the hepatic acute phase response but alsoin adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion.4 Overproduction of IL-6,a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline.5BSF-2 is a novel interleukin consisting of 184 amino acids.6
Mouse Interleukin-6 Protein information
Uniprot ID IL6_MOUSE
Uniprot AC P08505; Q3UCQ0; Q8BN26;
UniGene Mm.1019;
GeneID 16193
KEGG mmu:16193;
Mouse Interleukin-6 Protein SEQUENCE
SEQUENCE 211 AA; 24384 MW; BBB47DDA9E86787A CRC64;
MKFLSARDFH PVAFLGLMLV TTTAFPTSQV RRGDFTEDTT PNRPVYTTSQ
VGGLITHVLW EIVEMRKELC NGNSDCMNND DALAENNLKL PEIQRNDGCY
QTGYNQEICL LKISSGLLEY HSYLEYMKNN LKDNKKDKAR VLQRDTETLI
HIFNQEVKDL HKIVLPTPIS NALLTDKLES QKEWLRTKTI QFILKSLEEF
LKVTLRSTRQ T
UCSC uc008wuu.1; mouse.;

Category

Mouse Il6 ELISA Kit

Mouse Il-6 ELISA Kit;Mouse B-cell hybridoma growth factor ELISA Kit;Mouse interleukin HP-1 ELISA Kit;Mouse interleukin 6 ELISA Kit;Mouse interleukin-6 ELISA Kit;