SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse INSL5. No significant cross-reactivity or interference between Mouse INSL5 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse INSL5 and all the analogues, therefore, cross reaction may still exist.
Intended UseMouse Insl5 ELISA Kit allows for the in vitro quantitative determination of Insl5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of INSL5 in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse INSL5. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse INSL5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INSL5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse INSL5. You can calculate the concentration of Mouse INSL5 in the samples by comparing the OD of the samples to the standard curve.
Mouse Insulin-like peptide INSL5 A chain Protein information
Uniprot ID
INSL5_MOUSE
Uniprot AC
Q9WUG6;
UniGene
Mm.100163;
GeneID
23919
KEGG
mmu:23919;
Mouse Insulin-like peptide INSL5 A chain Protein SEQUENCE