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Mouse FIL1d ELISA Kit

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  • Alternative name

    Interleukin-36 receptor antagonist protein ELISA KIT; FIL1 delta ELISA KIT; IL-1-related protein 3 ELISA KIT; IL-1RP3 ELISA KIT; Interleukin-1 HY1 ELISA KIT; IL-1HY1 ELISA KIT; Interleukin-1 delta ELISA KIT; IL-1 delta ELISA KIT; Interleukin-1 family member 5 ELISA KIT; IL-1F5 ELISA KIT; Interleukin-1 receptor antagonist homolog 1 ELISA KIT; IL-1ra homolog 1 ELISA KIT; Interleukin-1-like protein 1 ELISA KIT; IL-1L1IL36RN ELISA KIT; FIL1D ELISA KIT; IL1F5 ELISA KIT; IL1HY1 ELISA KIT; IL1L1 ELISA KIT; IL1RP3 ELISA KIT; IL-1RP3 ELISA KIT; IL-1HY1 ELISA KIT; IL-1 delta ELISA KIT; IL-1F5 ELISA KIT; IL-1ra homolog 1 ELISA KIT; IL-1L1 ELISA KIT
  • Catalog
    E057111
  • species
    Mouse
  • GeneFIL1d
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<18.75pg/ml
  • Intended UseMouse FIL1d ELISA Kit allows for the in vitro quantitative determination of FIL1d , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse FIL1d antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse FIL1d antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse FIL1d amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse FIL1d can be calculated.



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