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Mouse FIL1h ELISA Kit

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  • Alternative name

    Interleukin-36 beta ELISA KIT; FIL1 eta ELISA KIT; Interleukin-1 eta ELISA KIT; IL-1 eta ELISA KIT; Interleukin-1 family member 8 ELISA KIT; IL-1F8 ELISA KIT; Interleukin-1 homolog 2 ELISA KIT; IL-1H2IL36B ELISA KIT; IL1F8 ELISA KIT; IL1H2 ELISA KIT; IL-1 eta ELISA KIT; IL-1F8 ELISA KIT; IL-1H2 ELISA KIT
  • Catalog
    E057116
  • species
    Mouse
  • GeneFIL1h
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseMouse FIL1h ELISA Kit allows for the in vitro quantitative determination of FIL1h , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical    Principle of the Assay: FIL1H ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FIL1H antibody and an FIL1H-HRP conjugate. The assay sample and buffer are incubated together with FIL1H-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FIL1H concentration since FIL1H from samples and FIL1H-HRP conjugate compete for the anti-FIL1H antibody binding site. Since the number of sites is limited, as more sites are occupied by FIL1H from the sample, fewer sites are left to bind FIL1H-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FIL1H concentration in each sample is interpolated from this standard curve.



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