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Mouse Il10ra ELISA Kit

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  • Alternative name

    Mouse IL-10 receptor subunit alpha ELISA Kit;Mouse IL-10R subunit alpha ELISA Kit;Mouse IL-10RA ELISA Kit;Mouse CDw210a ELISA Kit;Mouse interleukin-10 receptor subunit 1 ELISA Kit;Mouse IL-10R subunit 1 ELISA Kit;Mouse IL-10R1 ELISA Kit;Mouse Il10r ELISA Kit;Mouse AW553859 ELISA Kit;Mouse CDw210 ELISA Kit;Mouse mIL-10R ELISA Kit;Mouse interleukin 10 receptor, alpha ELISA Kit;Mouse interleukin-10 receptor subunit alpha ELISA Kit;
  • Catalog
    E057160
  • species
    Mouse
  • GeneIl10ra
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 10 Receptor Alpha (IL10Ra). No significant cross-reactivity or interference between Interleukin 10 Receptor Alpha (IL10Ra) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 30pg/mL.
  • Intended UseMouse Il10ra ELISA Kit allows for the in vitro quantitative determination of Il10ra , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 Receptor Alpha (IL10Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 10 Receptor Alpha (IL10Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 10 Receptor Alpha (IL10Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 10 Receptor Alpha (IL10Ra) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Interleukin-10 receptor subunit alpha Protein information
  • Uniprot ID I10R1_MOUSE
  • Uniprot AC Q61727;
  • UniGene Mm.379327;
  • GeneID 16154
  • KEGG mmu:16154;
  • Mouse Interleukin-10 receptor subunit alpha Protein SEQUENCE

  • SEQUENCE 575 AA; 64248 MW; 820B9CD576F686B7 CRC64;

    MLSRLLPFLV TISSLSLEFI AYGTELPSPS YVWFEARFFQ HILHWKPIPN

    QSESTYYEVA LKQYGNSTWN DIHICRKAQA LSCDLTTFTL DLYHRSYGYR

    ARVRAVDNSQ YSNWTTTETR FTVDEVILTV DSVTLKAMDG IIYGTIHPPR

    PTITPAGDEY EQVFKDLRVY KISIRKFSEL KNATKRVKQE TFTLTVPIGV

    RKFCVKVLPR LESRINKAEW SEEQCLLITT EQYFTVTNLS ILVISMLLFC

    GILVCLVLQW YIRHPGKLPT VLVFKKPHDF FPANPLCPET PDAIHIVDLE

    VFPKVSLELR DSVLHGSTDS GFGSGKPSLQ TEESQFLLPG SHPQIQGTLG

    KEESPGLQAT CGDNTDSGIC LQEPGLHSSM GPAWKQQLGY THQDQDDSDV

    NLVQNSPGQP KYTQDASALG HVCLLEPKAP EEKDQVMVTF QGYQKQTRWK

    AEAAGPAECL DEEIPLTDAF DPELGVHLQD DLAWPPPALA AGYLKQESQG

    MASAPPGTPS RQWNQLTEEW SLLGVVSCED LSIESWRFAH KLDPLDCGAA

    PGGLLDSLGS NLVTLPLISS LQVEE

  • UCSC uc009pfn.1; mouse.;



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