Mouse Idh2 ELISA Kit

Alternative name
Mouse IDH ELISA Kit;Mouse ICD-M ELISA Kit;Mouse IDP ELISA Kit;Mouse NADP(+)-specific ICDH ELISA Kit;Mouse oxalosuccinate decarboxylase ELISA Kit;Mouse E430004F23 ELISA Kit;Mouse IDPm ELISA Kit;Mouse Idh-2 ELISA Kit;Mouse isocitrate dehydrogenase 2 (NADP+), mitochondrial ELISA Kit;Mouse isocitrate dehydrogenase [NADP], mitochondrial ELISA Kit;Mouse NADP+-specific isocitrate dehydrogenase ELISA Kit;
Catalog
E057603
species
Mouse
GeneIdh2
SpecificityThis assay has high sensitivity and excellent specificity for detection of IDH2. No significant cross-reactivity or interference between IDH2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IDH2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Idh2 ELISA Kit allows for the in vitro quantitative determination of Idh2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: IDH2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IDH2 antibody and an IDH2-HRP conjugate. The assay sample and buffer are incubated together with IDH2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IDH2 concentration since IDH2 from samples and IDH2-HRP conjugate compete for the anti-IDH2 antibody binding site. Since the number of sites is limited, as more sites are occupied by IDH2 from the sample, fewer sites are left to bind IDH2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IDH2 concentration in each sample is interpolated from this standard curve.
Mouse Isocitrate dehydrogenase [NADP], mitochondrial Protein information
Uniprot ID IDHP_MOUSE
Uniprot AC P54071; Q8C2R9; Q9EQK1;
UniGene Mm.246432; Mm.290925;
GeneID 269951
KEGG mmu:269951;
Mouse Isocitrate dehydrogenase [NADP], mitochondrial Protein SEQUENCE
SEQUENCE 452 AA; 50906 MW; 19BB5CF78512ECAB CRC64;
MAGYLRAVSS LCRASGSART WAPAALTVPS WPEQPRRHYA EKRIKVEKPV
VEMDGDEMTR IIWQFIKEKL ILPHVDVQLK YFDLGLPNRD QTNDQVTIDS
ALATQKYSVA VKCATITPDE ARVEEFKLKK MWKSPNGTIR NILGGTVFRE
PIICKNIPRL VPGWTKPITI GRHAHGDQYK ATDFVVDRAG TFKLVFTPKD
GSSAKEWEVY NFPAGGVGMG MYNTDESISG FAHSCFQYSI QKKWPLYLST
KNTILKAYDG RFKDIFQEIF DKHYKTDFDK NKIWYEHRLI DDMVAQVLKS
SGGFVWACKN YDGDVQSDIL AQGFGSLGLM TSVLVCPDGK TIEAEAAHGT
VTRHYREHQK GRPTSTNPIA SIFAWTRGLE HRGKLDGNQD LIRFAQTLEK
VCVQTVESGA MTKDLAGCIH GLSNVKLNEH FLNTTDFLDT IKSNLDRALG
KQ
UCSC uc009hzm.2; mouse.;

Category

Mouse Idh2 ELISA Kit