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Catalog
E058462
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species
Mouse
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GeneLeptin Ab
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Leptin-Ab. No significant cross-reactivity or interference between Leptin-Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Leptin-Ab and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ug/mL
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Intended UseMouse Leptin Ab ELISA Kit allows for the in vitro quantitative determination of Leptin Ab , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyImmunology
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Product Description
specificalIntended Uses||This Leptin-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse Leptin-Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: Leptin-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Leptin antigen and an Leptin-Ab-HRP conjugate. The assay sample and buffer are incubated together with Leptin-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Leptin-Ab concentration since Leptin-Ab from samples and Leptin-Ab-HRP conjugate compete for the Leptin antigen binding site. Since the number of sites is limited, as more sites are occupied by Leptin-Ab from the sample, fewer sites are left to bind Leptin-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Leptin-Ab concentration in each sample is interpolated from this standard curve.
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