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Mouse Lif ELISA Kit

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  • Alternative name

    Mouse LIF ELISA Kit;Mouse differentiation-stimulating factor ELISA Kit;Mouse d factor ELISA Kit;Mouse leukemia inhibitory factor ELISA Kit;Mouse myeloid leukaemia inhibitory factor ELISA Kit;
  • Catalog
    E058617
  • species
    Mouse
  • GeneLif
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<46.9pg/ml
  • Intended UseMouse Lif ELISA Kit allows for the in vitro quantitative determination of Lif , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Lif antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Lif antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Lif amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Lif can be calculated.
  • Mouse Leukemia inhibitory factor Protein information
  • Uniprot ID LIF_MOUSE
  • Uniprot AC P09056;
  • UniGene Mm.4964;
  • GeneID 16878
  • KEGG mmu:16878;
  • Mouse Leukemia inhibitory factor Protein SEQUENCE

  • SEQUENCE 203 AA; 22287 MW; F5C65EF11A67A835 CRC64;

    MKVLAAGIVP LLLLVLHWKH GAGSPLPITP VNATCAIRHP CHGNLMNQIK

    NQLAQLNGSA NALFISYYTA QGEPFPNNVE KLCAPNMTDF PSFHGNGTEK

    TKLVELYRMV AYLSASLTNI TRDQKVLNPT AVSLQVKLNA TIDVMRGLLS

    NVLCRLCNKY RVGHVDVPPV PDHSDKEAFQ RKKLGCQLLG TYKQVISVVV

    QAF

  • UCSC uc007hut.1; mouse.;



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