Mouse Madcam1 ELISA Kit

Alternative name
Mouse MAdCAM-1 ELISA Kit;Mouse mMAdCAM-1 ELISA Kit;Mouse AV211525 ELISA Kit;Mouse mucosal vascular addressin cell adhesion molecule 1 ELISA Kit;Mouse mucosal addressin cell adhesion molecule 1 ELISA Kit;Mouse cell adhesion molecule 1 ELISA Kit;
Catalog
E059317
species
Mouse
GeneMadcam1
SpecificityNatural and recombinant mouse MADCAM-1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseMouse Madcam1 ELISA Kit allows for the in vitro quantitative determination of Madcam1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse MADCAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MADCAM-1 has been precoated onto 96-well plates. Standards(NSO, Q22-T365) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MADCAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MADCAM-1 amount of sample captured in plate. Background: MADCAM1(Mucosal Vascular Addressin Cell Adhesion Molecule 1), also known as MACAM1, is a protein that is encoded by the MADCAM1 gene. By PCR-based analysis of somatic cell hybrids, Leung et al(1997) mapped the MACAM1 gene to chromosome 19. The protein encoded by this gene is an endothelil cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1(alpha4/beta7), L-selectin, and VLA-4(alpha4/beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin superfamily and is similar to ICAM-1 and VCAM-1.
Mouse Mucosal addressin cell adhesion molecule 1 Protein information
Uniprot ID MADCA_MOUSE
Uniprot AC Q61826; O35530; Q61278; Q64275; Q8R1M6;
UniGene Mm.391556;
GeneID 17123
Mouse Mucosal addressin cell adhesion molecule 1 Protein SEQUENCE
SEQUENCE 405 AA; 43652 MW; 4F2D08418EA51980 CRC64;
MESILALLLA LALVPYQLSR GQSFQVNPPE SEVAVAMGTS LQITCSMSCD
EGVARVHWRG LDTSLGSVQT LPGSSILSVR GMLSDTGTPV CVGSCGSRSF
QHSVKILVYA FPDQLVVSPE FLVPGQDQVV SCTAHNIWPA DPNSLSFALL
LGEQRLEGAQ ALEPEQEEEI QEAEGTPLFR MTQRWRLPSL GTPAPPALHC
QVTMQLPKLV LTHRKEIPVL QSQTSPKPPN TTSAEPYILT SSSTAEAVST
GLNITTLPSA PPYPKLSPRT LSSEGPCRPK IHQDLEAGWE LLCEASCGPG
VTVRWTLAPG DLATYHKREA GAQAWLSVLP PGPMVEGWFQ CRQDPGGEVT
NLYVPGQVTP NSSSTVVLWI GSLVLGLLAL VFLAYRLWKC YRPGPRPDTS
SCTHL

Category

Mouse Madcam1 ELISA Kit

Mouse MAdCAM-1 ELISA Kit;Mouse mMAdCAM-1 ELISA Kit;Mouse AV211525 ELISA Kit;Mouse mucosal vascular addressin cell adhesion molecule 1 ELISA Kit;Mouse mucosal addressin cell adhesion molecule 1 ELISA Kit;Mouse cell adhesion molecule 1 ELISA Kit;