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Alternative name
Tesmin ELISA KIT; Metallothionein-like 5, testis-specific ELISA KIT; Testis-specific metallothionein-like proteinMtl5 ELISA KIT;
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Catalog
E059962
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species
Mouse
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GeneMTL5
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SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse MTL5. No significant cross-reactivity or interference between mouse MTL5 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of mouse MTL5 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
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Intended UseMouse MTL5 ELISA Kit allows for the in vitro quantitative determination of MTL5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MTL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MTL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTL5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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