Mouse Smad7 ELISA Kit

Alternative name
Mouse MAD homolog 7 ELISA Kit;Mouse mothers against DPP homolog 7 ELISA Kit;Mouse mothers against decapentaplegic homolog 8 ELISA Kit;Mouse MAD homolog 8 ELISA Kit;Mouse mothers against DPP homolog 8 ELISA Kit;Mouse SMAD family member 7 ELISA Kit;Mouse SMAD 7 ELISA Kit;Mouse Smad7 ELISA Kit;Mouse Madh7 ELISA Kit;Mouse madh8 ELISA Kit;Mouse mothers against decapentaplegic homolog 7 ELISA Kit;
Catalog
E060475
species
Mouse
GeneSmad7
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseMouse Smad7 ELISA Kit allows for the in vitro quantitative determination of Smad7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Smad7 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Smad7 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Smad7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofMouse Smad7 can be calculated.
Mouse Mothers against decapentaplegic homolog 7 Protein information
Uniprot ID SMAD7_MOUSE
Uniprot AC O35253; O88709;
UniGene Mm.34407;
GeneID 17131
KEGG mmu:17131;
Mouse Mothers against decapentaplegic homolog 7 Protein SEQUENCE
SEQUENCE 426 AA; 46442 MW; BEEE751371C0E0CF CRC64;
MFRTKRSALV RRLWRSRAPG GEDEEEGVGG GGGGGELRGE GATDGRAYGA
GGGGAGRAGC CLGKAVRGAK GHHHPHPPTS GAGAAGGAEA DLKALTHSVL
KKLKERQLEL LLQAVESRGG TRTACLLLPG RLDCRLGPGA PASAQPAQPP
SSYSLPLLLC KVFRWPDLRH SSEVKRLCCC ESYGKINPEL VCCNPHHLSR
LCELESPPPP YSRYPMDFLK PTAGCPDAVP SSAETGGTNY LAPGGLSDSQ
LLLEPGDRSH WCVVAYWEEK TRVGRLYCVQ EPSLDIFYDL PQGNGFCLGQ
LNSDNKSQLV QKVRSKIGCG IQLTREVDGV WVYNRSSYPI FIKSATLDNP
DSRTLLVHKV FPGFSIKAFD YEKAYSLQRP NDHEFMQQPW TGFTVQISFV
KGWGQCYTRQ FISSCPCWLE VIFNSR
UCSC uc008fqe.2; mouse. [O35253-2];

Category

Mouse Smad7 ELISA Kit