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Mouse Muc2 ELISA Kit

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  • Alternative name

    Mouse MUC-2 ELISA Kit;Mouse Colonic mucin ELISA Kit;Mouse MCM ELISA Kit;Mouse Secreted gel-forming mucin ELISA Kit;
  • Catalog
    E060534
  • species
    Mouse
  • GeneMuc2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MUC2. No significant cross-reactivity or interference between MUC2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MUC2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Muc2 ELISA Kit allows for the in vitro quantitative determination of Muc2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Principle of the Assay: MUC2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MUC2 antibody and an MUC2-HRP conjugate. The assay sample and buffer are incubated together with MUC2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MUC2 concentration since MUC2 from samples and MUC2-HRP conjugate compete for the anti-MUC2 antibody binding site. Since the number of sites is limited, as more sites are occupied by MUC2 from the sample, fewer sites are left to bind MUC2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MUC2 concentration in each sample is interpolated from this standard curve.
  • Mouse Mucin-2 Protein information
  • Uniprot ID MUC2_MOUSE
  • Uniprot AC Q80Z19; Q0P637; Q80Z17; Q8K0Q1; Q9CVG8; Q9Z2U5;
  • UniGene Mm.2041; Mm.461296;
  • Mouse Mucin-2 Protein SEQUENCE

  • SEQUENCE 2680 AA; 293436 MW; D67D8664AAB15933 CRC64;

    MGLPLARLVA ACLVLALAKG SELQKEARSR NHVCSTWGDF HYKTFDGDVY

    RFPGLCDYNF ASDCRDSYKE FAVHLKRGLG EAGGHSQIES ILITIKDDTI

    YLTHKLAVVN GAMVSTPHYS SGLLIEKNDA YTKVYSRAGL SLMWNREDAL

    MVELDSRFQN HTCGLCGDFN GMQTNYEFLS EEGIQFSAIE FGNMQKINKP

    EVQCEDPEAV QEPESCSEHR AECERLLTSA AFEDCQTRVP VESYVRACMH

    DRCQCPKGGA CECSTLAEFS RQCSHAGGRP ENWRTASLCP KKCPNNMVYL

    ESSSPCVDTC SHLEVSSLCE EHYMDGCFCP EGTVYDDITG SGCIPVSQCH

    CKLHGHLYMP GQEFTNDCEQ CVCNAGRWVC KDLPCPETCA LEGGSHITTF

    DGKKFTFHGD CYYVLTKSEH NDSYALLGEL ASCGSTDKQT CLKTVVLLTD

    DKKNVVAFKS GGSVLLNEME VTLPHVAASF SIFQPSSYHI VVNTKFGLRL

    QIQLLPVMQL FVTLDQAAQG QVQGLCGNFN GLESDDFMTS GGMVEATGAG

    FANTWKAQSS CHDKLDWLDD PCSLNIETNY AEHWCSLLKR SETPFARCHL

    AVDPTEYYKR CKYDTCNCQN NEDCMCAALS SYARACAAKG VMLWGWRERV

    CNKDVHACPS SQIFMYNLTT CQQTCRSLSE GDSHCLKGFA PVEGCGCPDH

    TFMDEKGRCV PLAKCSCYHH GLYLEAGDVI LRQEERCICR NGRLQCTQVK

    LIGHTCQYPK ILVDCNNLTA LAVRKPRPTS CQTLVAGYYH TECISGCVCP

    DGLLDDGRGG CVEEDKCPCI HNKDLYSSGE SIKLDCNNTC TCQKGRWECT

    RYACHSTCSI YGSGHYITFD GKHYDFDGHC SYVAVQDYCG QNSTGSFSII

    TENVPCGTTG VTCSKAIKIF IGGTELKLVD KHRVVKQLEE GHHVPYITRE

    VGQYLVVEAS SGIIVIWDKK TTIFIKLDPS YKGTVCGLCG NFDDQTKNDF

    TTRDHMVVTS ELDFGNSWKE ASTCPDVSHN PDPCSLNPHR RSWAEKQCSI

    IKSRVFKVCH SKVDPTVFYE ACVHDSCSCD TGGDCDCFCS AVASYAQECT

    KAEACVFWRT PDLCPIFCDY YNPPDECEWH YEPCGNRSFE TCRTLNGIHS

    NISVSYLEGC YPRCPEDRPI YDEDLKKCVT GDKCGCYIED TRYPPGGSVP

    TDEICKSCTC TNTSKIECHP DEGKILNMTQ DGIFCYWEFC GPNGTVGQHF

    NICGSSTAIP STTTSFTTIS TPISTTPIST TITTTTVTMT TEQVPCCFWS

    DWINKYHPTK ENGGDRETFT HVCSAPEDIE CRAATDPKLS WEELGQKVQC

    NVSTGLICNN EDQYGIGEFE LCYDYEIRVN CCYPMEYCTP STISPTTSTT

    TLSTTPPTSS PTTLPTSSPV TSSATLPTTS SITSTISPTT SPSTATQTIS

    VTTSQTSSSA TPPNSSPTSS ATTSPTTSSG TSTATSPSTS PTTSSTFTTP

    PSTTCIDDCK WTGWLDSGKP TYDIKSGDFE LIKGVCEPHW EVQNISCRAV

    MHSNIPLDQL GQIVVCNKEV GLVCKNEDQE IGGIIPMRMC LNYEINVYCC

    NPICFTSTPS STTTETPTTT STTKTSILTS TTTQTPSPSP TTTVTPTPAP

    TTTQIPTSTS TTTQTTTPTP ITETSTPTST ISQTPSPAST TTVTPATTST

    TTETSTSTST TTQTTSPTPT VTETSTPRST TTQTPSPVPT TTVTSTPTPT

    IGETTTPKRP PSTSTPTSFT VPTETTTQTR PLSTTPTTLE TTRTSSWGTF

    SSTSPITSPS TVWTHTETQV TCCVLNEMFY GPGELVYNST HGGTCFYVNC

    SLDCHLQFFN WSCPSTPSTP TPSTPTPTPS QTTTPSTTSS KSTPSTPQST

    SPKSTLSTPT KTTPYGCPDF DPPRQVNETW WLCNCTMAIC NHDNVVEIVP

    LKCDPPPMPT CANGLKPVRV PDADNCCWHW ECDCYCTGWG DPHFVTFDGL

    YYSYQGNCTY VLVEEITPTV DNFGVYIDNY HCDANDKVSC PRTLIVRHET

    QEVQIKTVRM MPIEVEVQVN KQLVALPYKK YGLEVYESGI NIVVNISRLE

    AKISYNGLSF SIRLPYKLFV NNTKGQCGTC TNNTADDCIL PSGKIISDCE

    IAADEWLVND PSKPHCPHKG LTTKRPATTT PGLSLNNCTV SPVCHLIMDS

    LFSQCHAFVP PKHYYEACLF DSCYVPGSNM ECASVQAYAT LCAKEGVCID

    WRNHTQGVCS VKCPPHKQYQ ACGPEEEPTC QPSSSQNSTL LVEGCFCPEG

    TTKFAPGYDV CVKTCGCVGP DNVPREFGEH FEFDCKDCVC REGGSGIVCQ

    PKKCSGGNQT TCEEDGTYLV VETNPDDKCC NITSCKCDTK RCKAERPTCL

    LGFEVKTEIV PGKCCPVYSC VPKGVCVHQN AEYQPGSPVY SNKCQDCVCT

    NILDNSTQLN VISCTHVPCN ISCSSGFELV DVPGECCKKC QQTHCIIEGP

    KQQYIILKPG EIHKNPSNKC TFFSCMKINN QLISSVSNIT CPDFNPSDCV

    SGSITYMPNG CCKTCIPQNQ TRVPCSAVSV MKEISYNGCT KNISMNYCFG

    SCGTFAMYSA QVQGLDHRCS CCKEEKTSVR SVTLECPDGS ELSHTYTHIE

    SCLCQDTVCG LPQAQQVRTR RSSPRFLGRK

  • UCSC uc029wpp.2; mouse.;



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