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Catalog
E060744
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species
Mouse
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.156 ng/ml
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Intended UseMouse MPO-ANCA IgG ELISA Kit allows for the in vitro quantitative determination of , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with myeloperoxidase-antineutrophil cytoplasmic antigen. Standards or samples are then added to the appropriate microtiter plate wells with biotin-conjugated myeloperoxidase-antineutrophil cytoplasmic and Avidin conjugated to Horseradish Peroxidase (HRP) i s added to eachmicroplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain myeloperoxidase-antineutrophil cytoplasmic antibody, biotin-conjugated myeloperoxidase-antineutrophil cytoplasmic and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of myeloperoxidase-antineutrophil cytoplasmic antibody in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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