Mouse Nln ELISA Kit

Alternative name
Mouse microsomal endopeptidase ELISA Kit;Mouse MEP ELISA Kit;Mouse mitochondrial oligopeptidase M ELISA Kit;Mouse neurotensin endopeptidase ELISA Kit;Mouse 4930472G13Rik ELISA Kit;Mouse C79345 ELISA Kit;Mouse neurolysin (metallopeptidase M3 family) ELISA Kit;Mouse neurolysin, mitochondrial ELISA Kit;
Catalog
E061411
species
Mouse
GeneNln
SpecificityThis assay has high sensitivity and excellent specificity for detection of NLN. No significant cross-reactivity or interference between NLN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NLN and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Nln ELISA Kit allows for the in vitro quantitative determination of Nln , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: NLN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NLN antibody and an NLN-HRP conjugate. The assay sample and buffer are incubated together with NLN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NLN concentration since NLN from samples and NLN-HRP conjugate compete for the anti-NLN antibody binding site. Since the number of sites is limited, as more sites are occupied by NLN from the sample, fewer sites are left to bind NLN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NLN concentration in each sample is interpolated from this standard curve.
Mouse Neurolysin, mitochondrial Protein information
Uniprot ID NEUL_MOUSE
Uniprot AC Q91YP2; Q3UJP1; Q8R3F4;
UniGene Mm.127692;
GeneID 75805
KEGG mmu:75805;
Mouse Neurolysin, mitochondrial Protein SEQUENCE
SEQUENCE 704 AA; 80429 MW; E4F342E346AB0E07 CRC64;
MITLCLSALR GLHRAGGSRI RLRMTLGREA ASPLQAMSSY TAAGRNVLRW
DLSPEQIRTR TEELIAQTKQ VYDTVGTINL EDVTYENCLQ VLADIEVKYI
VERTMLDFPQ HVSSDREVRA ASTEADKRLS RFDIEMSMRE DVFQRIVHLQ
ETCDLEKIKP EARRYLEKSI KMGKRNGLHL PEHVKNEIKS MKKRMSELCI
DFNKNLNEDD TSLVFSKAEL GALPDDFIDS LEKTDEDKYK VTLKYPHYFP
VMKKCCVPET RRKMEMAFHT RCKEENTIIL QQLLPLRAQV AKLLGYNTHA
DFVLELNTAK STSHVATFLD DLSQKLKPLG EAEREFILSL KKKECEERGF
AYDGKINAWD LHYYMTQTEE LKYSVDQESL KEYFPIEVVT EGLLSIYQEL
LGLSFEQVAD AHVWNKSVSL YTVKDKATGE VLGQFYLDLY PREGKYNHAA
CFGLQPGCLL PDGSRMMSVA ALVVNFSQPI AGRPSLLRHD EVRTYFHEFG
HVMHQICAQT DFARFSGTNV ETDFVEVPSQ MLENWVWDID SLRKLSKHYR
DGHPITDELL EKLVASRLVN TGLLTLRQIV LSKVDQSLHT NASLDAASEY
AKYCTEILGV AATPGTNMPA TFGHLAGGYD GQYYGYLWSE VFSMDMFHSC
FRKEGIMNPE VGMKYRNLIL KPGGSLDGMD MLQNFLQREP NQKAFLMSRG
LNAS
UCSC uc007rsp.1; mouse.;

Category

Mouse Nln ELISA Kit