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Alternative name
Neutrophil gelatinase-associated lipocalin ELISA KIT; 25 kDa alpha-2-microglobulin-related subunit of MMP-9 ELISA KIT; Lipocalin-2 ELISA KIT; Oncogene 24p3 ELISA KIT; Siderocalin LCN2 ELISA KIT; p25LCN2 ELISA KIT; HNL ELISA KIT; NGAL ELISA KIT; NGAL ELISA KIT
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Catalog
E061572
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species
Mouse
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GeneNGAL
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Neutrophil Gelatinase Associated Lipocalin (NGAL).
No significant cross-reactivity or interference between Neutrophil Gelatinase Associated Lipocalin (NGAL) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.063ng/mL.
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Intended UseMouse NGAL ELISA Kit allows for the in vitro quantitative determination of NGAL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Gelatinase Associated Lipocalin (NGAL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neutrophil Gelatinase Associated Lipocalin (NGAL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutrophil Gelatinase Associated Lipocalin (NGAL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neutrophil Gelatinase Associated Lipocalin (NGAL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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