Mouse Nucb2 ELISA Kit

Alternative name
Mouse DNA-binding protein NEFA ELISA Kit;Mouse prepronesfatin ELISA Kit;Mouse Nefa ELISA Kit;Mouse AI607786 ELISA Kit;Mouse Calnuc ELISA Kit;Mouse Nesfatin-1 ELISA Kit;Mouse nucleobindin 2 ELISA Kit;Mouse nucleobindin-2 ELISA Kit;Mouse prepronefastin ELISA Kit;
Catalog
E061987
species
Mouse
GeneNucb2
SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse NUCB2. No significant cross-reactivity or interference between mouse NUCB2 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of mouse NUCB2 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseMouse Nucb2 ELISA Kit allows for the in vitro quantitative determination of Nucb2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NUCB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NUCB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NUCB2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NUCB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Nesfatin-1 Protein information
Uniprot ID NUCB2_MOUSE
Uniprot AC P81117;
UniGene Mm.9901;
GeneID 53322
KEGG mmu:53322;
Mouse Nesfatin-1 Protein SEQUENCE
SEQUENCE 420 AA; 50304 MW; 0DEBD51724CB7E72 CRC64;
MRWRIIQVQY CFLLVPCMLT ALEAVPIDVD KTKVHNTEPV ENARIEPPDT
GLYYDEYLKQ VIEVLETDPH FREKLQKADI EEIRSGRLSQ ELDLVSHKVR
TRLDELKRQE VGRLRMLIKA KLDALQDTGM NHHLLLKQFE HLNHQNPNTF
ESRDLDMLIK AATADLEQYD RTRHEEFKKY EMMKEHERRE YLKTLSEEKR
KEEESKFEEM KRKHEDHPKV NHPGSKDQLK EVWEETDGLD PNDFDPKTFF
KLHDVNNDGF LDEQELEALF TRELEKVYNP QNAEDDMIEM EEERLRMREH
VMSEIDNNKD RLVTLEEFLR ATEKKEFLEP DSWETLDQQQ LFTEDELKEY
ESIIAIQENE LKKRAEELQK QKEDLQRQHD HLEAQKQEYH QAVQHLEQKK
LQQGIAPSGP AGELKFEPHT
UCSC uc009jjk.3; mouse.;

Category

Mouse Nucb2 ELISA Kit

Mouse DNA-binding protein NEFA ELISA Kit;Mouse prepronesfatin ELISA Kit;Mouse Nefa ELISA Kit;Mouse AI607786 ELISA Kit;Mouse Calnuc ELISA Kit;Mouse Nesfatin-1 ELISA Kit;Mouse nucleobindin 2 ELISA Kit;Mouse nucleobindin-2 ELISA Kit;Mouse prepronefastin ELISA Kit;