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Alternative name
Orexin ELISA KIT; Hypocretin ELISA KIT; HcrtHCRT ELISA KIT; OX ELISA KIT; PPORX ELISA KIT; PPOX ELISA KIT; Hcrt ELISA KIT; Hcrt1 ELISA KIT; Hcrt2 ELISA KIT
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Catalog
E062591
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species
Mouse
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GeneOX
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Orexin (OX).
No significant cross-reactivity or interference between Orexin (OX) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 2.94pg/mL.
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Intended UseMouse OX ELISA Kit allows for the in vitro quantitative determination of OX , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Orexin (OX) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Orexin (OX) and unlabeled Orexin (OX) (Standards or samples) with the pre-coated antibody specific to Orexin (OX). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Orexin (OX) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Orexin (OX) in the sample.
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