Mouse Pick1 ELISA Kit

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  • Alternative name

    Mouse Protein interacting with C kinase 1 ELISA Kit;Mouse Protein kinase C-alpha-binding protein ELISA Kit;Mouse Prkcabp ELISA Kit;

  • Catalog
    E063772
  • species
    Mouse
  • GenePick1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse PICK1. No significant cross-reactivity or interference between Mouse PICK1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse PICK1 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Pick1 ELISA Kit allows for the in vitro quantitative determination of Pick1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PICK1 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PICK1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse PICK1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PICK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse PICK1. You can calculate the concentration of Mouse PICK1 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse PRKCA-binding protein Protein information
  • Uniprot ID PICK1_MOUSE
  • Uniprot AC Q62083; E9PY04;
  • UniGene Mm.259464;
  • Mouse PRKCA-binding protein Protein SEQUENCE
  • SEQUENCE 416 AA; 46597 MW; 437369D0AB7DCE7B CRC64;

    MFADLDYDIE EDKLGIPTVP GKVTLQKDAQ NLIGISIGGG AQYCPCLYIV

    QVFDNTPAAL DGTVAAGDEI TGVNGKSIKG KTKVEVAKMI QEVKGEVTIH

    YNKLQADPKQ GMSLDIVLKK VKHRLVENMS SGTADALGLS RAILCNDGLV

    KRLEELERTA ELYKGMTEHT KNLLRAFYEL SQTHRAFGDV FSVIGVREPQ

    PAASEAFVKF ADAHRSIEKF GIRLLKTIKP MLTDLNTYLN KAIPDTRLTI

    KKYLDVKFEY LSYCLKVKEM DDEEYSCIGP RRALYRVSTG NYEYRLILRC

    RQEARARFSQ MRKDVLEKME LLDQKHVQDI VFQLQRFVST MSKYYNDCYA

    VLQDADVFPI EVDLAHTTLA YGPNQGSFTD GEEEDEEEED GAAREVSKDA

    CGATGPTDKG GSWCDS




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