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Alternative name
Tissue-type plasminogen activator ELISA KIT; INN: Alteplase ELISA KIT; INN: ReteplaseCleaved into the following 2 chains:Tissue-type plasminogen activator chain A ELISA KIT; Tissue-type plasminogen activator chain BPLAT ELISA KIT; t-PA ELISA KIT; t-plasminogen activator ELISA KIT; tPA ELISA KIT
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Catalog
E063903
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species
Mouse
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GenePLGA
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseMouse PLGA ELISA Kit allows for the in vitro quantitative determination of PLGA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilySignal Transduction
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Product Description
specificalPrinciple of the Assay: PLGA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PLGA antibody and an PLGA-HRP conjugate. The assay sample and buffer are incubated together with PLGA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PLGA concentration since PLGA from samples and PLGA-HRP conjugate compete for the anti-PLGA antibody binding site. Since the number of sites is limited, as more sites are occupied by PLGA from the sample, fewer sites are left to bind PLGA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLGA concentration in each sample is interpolated from this standard curve.
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