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Alternative name
Podocin ELISA KIT; NPHS2 ELISA KIT;
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Catalog
E064144
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species
Mouse
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GenePDCN
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SpecificityThis assay has high sensitivity and excellent specificity for detection of podocin. No significant cross-reactivity or interference between podocin and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of podocin is typically less than 0.052ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding
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Intended UseMouse PDCN ELISA Kit allows for the in vitro quantitative determination of PDCN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to podocin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to podocin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain podocin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of podocin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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