SpecificityThis assay has high sensitivity and excellent specificity for detection of PFDN5. No significant cross-reactivity or interference between PFDN5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PFDN5 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseMouse Pfdn5 ELISA Kit allows for the in vitro quantitative determination of Pfdn5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description specificalIntended Uses||This PFDN5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PFDN5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: PFDN5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-PFDN5 antibody and an PFDN5-HRP conjugate. The assay sample and buffer are incubated together with PFDN5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PFDN5 concentration since PFDN5 from samples and PFDN5-HRP conjugate compete for the anti-PFDN5 antibody binding site. Since the number of sites is limited, as more sites are occupied by PFDN5 from the sample, fewer sites are left to bind PFDN5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PFDN5 concentration in each sample is interpolated from this standard curve.