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Mouse Psme2 ELISA Kit

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  • Alternative name

    Mouse 11S regulator complex subunit beta ELISA Kit;Mouse REG-beta ELISA Kit;Mouse Activator of multicatalytic protease subunit 2 ELISA Kit;Mouse Proteasome activator 28 subunit beta ELISA Kit;Mouse PA28b ELISA Kit;Mouse PA28beta ELISA Kit;Mouse Pa28b1 ELISA Kit;Mouse PA28b-p ELISA Kit;Mouse PA28b2 ELISA Kit;Mouse Psme2-like ELISA Kit;Mouse Psme2b-ps ELISA Kit;Mouse protease (prosome, macropain) activator subunit 2B ELISA Kit;Mouse protease (prosome, macropain) 28 subunit beta B ELISA Kit;Mouse OTTMUSWSBG00059279 ELISA Kit;Mouse OTTMUSWSBP00075795 ELISA Kit;Mouse PA28 beta subunit (PA28b) pseudogene ELISA Kit;Mouse protease (prosome, macropain) 28 subunit beta B, pseudogene ELISA Kit;Mouse protease (prosome, macropain) 28 subunit, beta, b ELISA Kit;Mouse proteasome activator complex like ELISA Kit;
  • Catalog
    E065009
  • species
    Mouse
  • GenePsme2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PSME2. No significant cross-reactivity or interference between PSME2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PSME2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Psme2 ELISA Kit allows for the in vitro quantitative determination of Psme2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical    Principle of the assay: PSME2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PSME2 antibody and an PSME2-HRP conjugate. The assay sample and buffer are incubated together with PSME2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PSME2 concentration since PSME2 from samples and PSME2-HRP conjugate compete for the anti-PSME2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PSME2 from the sample, fewer sites are left to bind PSME2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PSME2 concentration in each sample is interpolated from this standard curve.
  • Mouse Proteasome activator complex subunit 2 Protein information
  • Uniprot ID PSME2_MOUSE
  • Uniprot AC P97372; O35562;
  • UniGene Mm.371573; Mm.440151; Mm.441306;
  • GeneID 621823
  • KEGG mmu:621823;
  • Mouse Proteasome activator complex subunit 2 Protein SEQUENCE

  • SEQUENCE 239 AA; 27057 MW; D8BB90F78CB0DD7C CRC64;

    MAKPCGVRLS GEARKQVDVF RQNLFQEADD FLCTFLPRKI ISLSQLLQED

    SLNVADLSSL RAPLDIPIPD PPPKDDEMET DKQEKKEVPK CGYLPGNEKL

    LALLALVKPE VWTLKEKCIL VITWIQHLIP KIEDGNDFGV AIQEKVLERV

    NAVKTKVEAF QTTISKYFSE RGDAVAKASK DTHVMDYRAL VHERDEAAYG

    ALRAMVLDLR AFYAELYHII SSNLEKIVNP KGEEKPSMY

  • UCSC uc007tzg.1; mouse.;



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