Mouse Wnt3a ELISA Kit

Alternative name
Mouse Wnt-3a ELISA Kit;Mouse vt ELISA Kit;Mouse wingless-type MMTV integration site family, member 3A ELISA Kit;Mouse protein Wnt-3a ELISA Kit;Mouse vestigial tail ELISA Kit;Mouse wingless-related MMTV integration site 3A ELISA Kit;
Catalog
E065386
species
Mouse
GeneWnt3a
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse Wnt-3a. No significant cross-reactivity or interference between Mouse Wnt-3a and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Wnt3a ELISA Kit allows for the in vitro quantitative determination of Wnt3a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Wnt-3a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Wnt-3a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Wnt-3a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Wnt-3a can be calculated.
Mouse Protein Wnt-3a Protein information
Uniprot ID WNT3A_MOUSE
Uniprot AC P27467;
UniGene Mm.1367;
GeneID 22416
KEGG mmu:22416;
Mouse Protein Wnt-3a Protein SEQUENCE
SEQUENCE 352 AA; 39258 MW; 7ADFC5B38A8EFF63 CRC64;
MAPLGYLLVL CSLKQALGSY PIWWSLAVGP QYSSLSTQPI LCASIPGLVP
KQLRFCRNYV EIMPSVAEGV KAGIQECQHQ FRGRRWNCTT VSNSLAIFGP
VLDKATRESA FVHAIASAGV AFAVTRSCAE GSAAICGCSS RLQGSPGEGW
KWGGCSEDIE FGGMVSREFA DARENRPDAR SAMNRHNNEA GRQAIASHMH
LKCKCHGLSG SCEVKTCWWS QPDFRTIGDF LKDKYDSASE MVVEKHRESR
GWVETLRPRY TYFKVPTERD LVYYEASPNF CEPNPETGSF GTRDRTCNVS
SHGIDGCDLL CCGRGHNART ERRREKCHCV FHWCCYVSCQ ECTRVYDVHT
CK
UCSC uc007jdp.2; mouse.;

Category

Mouse Wnt3a ELISA Kit

Mouse Wnt-3a ELISA Kit;Mouse vt ELISA Kit;Mouse wingless-type MMTV integration site family, member 3A ELISA Kit;Mouse protein Wnt-3a ELISA Kit;Mouse vestigial tail ELISA Kit;Mouse wingless-related MMTV integration site 3A ELISA Kit;