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Alternative name
Mitochondrial chaperone BCS1 ELISA KIT; BCS1-like proteinBCS1L ELISA KIT; BCS1 ELISA KIT; h-BCS1 ELISA KIT
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Catalog
E065515
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species
Mouse
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GenePTD
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse PTD. No significant cross-reactivity or interference between Mouse PTD and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse PTD and all the analogues, therefore, cross reaction may still exist.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity3.75ng/mL
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Intended UseMouse PTD ELISA Kit allows for the in vitro quantitative determination of PTD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalDescription: The kit is a competitive enzyme immunoassay for in vitro quantitative measurement of PTD in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Mouse PTD. During the reaction, Mouse PTD in the sample or standard competes with a fixed amount of Mouse PTD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse PTD. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Mouse PTD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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