Mouse Pdk4 ELISA Kit

Alternative name
Mouse Pyruvate dehydrogenase kinase isoform 4 ELISA Kit;Mouse AV005916 ELISA Kit;Mouse pyruvate dehydrogenase kinase, isoenzyme 4 ELISA Kit;Mouse [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial ELISA Kit;Mouse [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial ELISA Kit;Mouse pyruvate dehydrogenase kinase 4 ELISA Kit;
Catalog
E065674
species
Mouse
GenePdk4
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseMouse Pdk4 ELISA Kit allows for the in vitro quantitative determination of Pdk4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse PDK4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse PDK4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PDK4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse PDK4 can be calculated.
Mouse [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial Protein information
Uniprot ID PDK4_MOUSE
Uniprot AC O70571;
UniGene Mm.235547;
GeneID 27273
KEGG mmu:27273;
Mouse [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial Protein SEQUENCE
SEQUENCE 412 AA; 46596 MW; 74815502E711054C CRC64;
MKAARFVMRS ASSLSSASLV PREVELFSRY SPSPLSMKQL LDFGSENACE
RTSFAFLRQE LPVRLANILK EIDILPDRLV NTPSVQLVKS WYIQSLMDLV
EFHEKSPEDQ KALSEFVDTL VKVRNRHHNV VPTMAQGILE YKDTCTVDPV
TNQNLQYFLD RFYMNRISTR MLMNQHILIF SDSKTGNPSH IGSIDPNCDV
VAVVQDAFEC AKMLCDQYYL TSPELNLTQV NGKFPGQPIH IVYVPSHLHH
MLFELFKNAM RATVEHQENR PSLTPVEATV VLGKEDLTIK ISDRGGGVPL
RITDRLFSYT YSTAPTPVMD NSRNAPLAGF GYGLPISRLY AKYFQGDLNL
YSMSGYGTDA IIYLKALSSE SVEKLPVFNK SAFKHYQMSS EADDWCIPSR
EPKNLAKEKL AV
UCSC uc009awk.1; mouse.;

Category

Mouse Pdk4 ELISA Kit