Mouse Akt1 ELISA Kit

Alternative name
Mouse AKT1 kinase ELISA Kit;Mouse Protein kinase B ELISA Kit;Mouse PKB ELISA Kit;Mouse Protein kinase B alpha ELISA Kit;Mouse PKB alpha ELISA Kit;Mouse proto-oncogene c-AKT ELISA Kit;Mouse RAC-PK-alpha ELISA Kit;Mouse Thymoma viral proto-oncogene ELISA Kit;Mouse Akt ELISA Kit;Mouse Rac ELISA Kit;Mouse LTR-akt ELISA Kit;Mouse PKB/Akt ELISA Kit;Mouse PKBalpha ELISA Kit;Mouse thymoma viral proto-oncogene 1 ELISA Kit;Mouse RAC-alpha serine/threonine-protein kinase ELISA Kit;Mouse Akt1m protein ELISA Kit;Mouse protein kinase B-alpha ELISA Kit;Mouse related to A and C kinases ELISA Kit;Mouse tumor rejection antigen peptide pRLib ELISA Kit;
Catalog
E065813
species
Mouse
GeneAkt1
SpecificityThis assay recognizes recombinant and natural mouse RAC-PK-alpha. No significant cross-reactivity or interference was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Akt1 ELISA Kit allows for the in vitro quantitative determination of Akt1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAC-PK-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RAC-PK-alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RAC-PK-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of RAC-PK-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse RAC-alpha serine/threonine-protein kinase Protein information
Uniprot ID AKT1_MOUSE
Uniprot AC P31750; Q62274; Q6GSA6;
UniGene Mm.6645;
GeneID 11651
KEGG mmu:11651;
Mouse RAC-alpha serine/threonine-protein kinase Protein SEQUENCE
SEQUENCE 480 AA; 55707 MW; 98DF28E5EFE03730 CRC64;
MNDVAIVKEG WLHKRGEYIK TWRPRYFLLK NDGTFIGYKE RPQDVDQRES
PLNNFSVAQC QLMKTERPRP NTFIIRCLQW TTVIERTFHV ETPEEREEWA
TAIQTVADGL KRQEEETMDF RSGSPSDNSG AEEMEVSLAK PKHRVTMNEF
EYLKLLGKGT FGKVILVKEK ATGRYYAMKI LKKEVIVAKD EVAHTLTENR
VLQNSRHPFL TALKYSFQTH DRLCFVMEYA NGGELFFHLS RERVFSEDRA
RFYGAEIVSA LDYLHSEKNV VYRDLKLENL MLDKDGHIKI TDFGLCKEGI
KDGATMKTFC GTPEYLAPEV LEDNDYGRAV DWWGLGVVMY EMMCGRLPFY
NQDHEKLFEL ILMEEIRFPR TLGPEAKSLL SGLLKKDPTQ RLGGGSEDAK
EIMQHRFFAN IVWQDVYEKK LSPPFKPQVT SETDTRYFDE EFTAQMITIT
PPDQDDSMEC VDSERRPHFP QFSYSASGTA
UCSC uc007pex.2; mouse.;

Category

Mouse Akt1 ELISA Kit